Chapter 1 Using microelectrodes
نویسنده
چکیده
Microelectrodes are the basis of the techniques discussed in this book. They are used for: (1) potential recording; (2) current injection; (3) introduction into the cell of ionselective resins for measuring potential or determining the free concentration of cytosolic constituents. (1) and (2) are the procedures underlying conventional microelectrode recording, voltage-clamping and patch-clamping. This chapter will be restricted to considering the fundamentals of reliable and accurate measurement of membrane potentials and the experimental manipulation of membrane potential by injection of current. The specialized techniques involved in voltage-clamping and patch-clamping will be treated separately in subsequent chapters, as will the special requirements involved in the use of ion-sensitive electrodes or ionophoresis of drugs.
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